How Do You Customise Database For Blast

  • 28 Feb, 2012

    Step 1. Assemble your sequences. Put all your sequences in Fasta format in a single file. This file should be located in a suitably named subdirectory of your home directory on the UBiC Blast server. The definition line for each sequence should start with a unique identifier for that sequence. Step 2. Convert this sequence file to a Blastable database.The command formatdb converts your Fasta file of sequences to a set of files that can be queried with command-line BLAST. Step 3. Test Blast on your database.See the UBiC tutorial, Using Command-line BLAST. In the blastall command-line you will need to specify the location of your database by typing: -d /disk2/home/myhome/blastdbs/custom.aa.

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